Figure 6. TGFβ induction of AREG in IECs is MEK1/2-dependent.
MSIE cells were treated with or without TGFβ. (A) The phosphorylated ERK1/2 (pERK1/2) was determined by Western blot (1 h). (B and C) MEK1/2 was inhibited or knockdown in MSIE cells in the presence of TGFβ. mRNA expression level of AREG was measured by qRT-PCR in the MSIE cells treated with (B) specific inhibitors or (C) siRNA. Data are presented as mean ± SEM of three independent experiments; NT, no treatment; NT siRNA, non-targeting siRNA. *P < 0.05, **P < 0.01, ***P < 0.001 one-way ANOVA.