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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: J Immunol. 2018 Aug 31;201(8):2452–2461. doi: 10.4049/jimmunol.1800537

Figure 5. Exposure to high density ICAM-1 activates IFN-γ production by a small subset of human lymphocytes directly ex vivo.

Figure 5.

A) Freshly isolated human PBMCs were incubated in culture wells coated with 5 μg/ml ICAM-1-Fc or with an isotype-matched negative control mAb, then subjected to flow cytometric analysis to assess CD11a expression levels and intracellular IFN-γ. Plots show results from analysis of the total lymphocyte gate as assessed by forward and side scatter. B) Aggregated results from analyses of PBMCs from six unrelated healthy adults, showing CD11a geometric mean fluorescence intensity (gMFI) for the lymphocyte population that stained positively for IFN-γ compared to those that did not. C and D) Freshly isolated human PBMCs were incubated in culture wells coated with 5 μg/ml ICAM-1-Fc in the presence of 5μM CsA (panel C) or 5μM U0126 (panel D), or vehicle alone, and then subjected to flow cytometric analysis to assess CD11a expression levels and intracellular IFN-γ.