Figure 1.

Expression Profiles of Developing Neurons Reveal a Dynamic Transcriptional Landscape

(A) Schematic representation of MB γ neuron remodeling and its regulation by the nuclear receptor (NR) complexes. The time-points taken for RNA-seq are indicated below. L2 and L3 refer to 2nd and 3rd instar larva, respectively.

(B) Schematic representation of the strategy we employed to isolate γ neurons from fly brains at different developmental stages.

(C) Global correlation matrix of the expression profiles indicated. We sequenced MB γ neurons at 14 developmental stages, as well as adult α/β MB neurons (labeled using NP3061-Gal4), non-MB neurons (labeled by c155-Gal4 combined with MB247-Gal80), and astrocyte-like cells (labeled by alrm-Gal4; marked as Glia).

(D) Principal-component analysis (PCA) of the neural expression profiles shown in (C). Numbers indicate hours after puparium formation (APF).