Figure 2.

Developmental Expression Atlas Highlights Axon Remodeling Gene Network

(A) Schematic description of the developmental RNA-seq gating analysis. See details in STAR Methods.

(B) Normalized expression of key remodeling related genes across γ neuron development (x axis, only every second time point is labeled due to space limitations; Ad, Adult). Error bars indicate SEM; units on the y axis are arbitrary.

(C) Heatmap showing k-means clustering (k = 10) of 2,671 dynamically expressed genes across MB γ neuron development. Each horizontal line describes the relative expression of a single gene. Cluster numbers are indicated using Roman numerals. Selected enriched processes within each cluster are described on the right (for a full analysis, see Tables S2 and S3). Enrichment is described here as the proportion of genes within the cluster/total number of genes belonging to the functional group and that are significantly expressed.

(D) Examples of functional groups of genes that are expressed in a similar, and interesting, pattern. Heatmap depicting the relative expression patterns of the proteasome subunits (left). The magenta scale depicts the peak expression of each gene relative to that of other genes in the group. Graphs showing the normalized expression levels of selected genes and gene groups throughout development (x axis, every second time point is labeled due to space limitations; Ad, Adult). Error bars indicate SEM; units on the y axis are arbitrary. Cluster numbers are indicated in parenthesis using Roman numerals.