Figure 2.

Epigenetic Priming of Lineage- and Stage-Specific Regulatory Elements before Their Activation during Astrogliogenesis

(A–C) UCSC browser track showing H3K4me1 enrichment at the regulatory regions of astroglial genes (A: Gfap, B: Aqp4) and neuronal gene (C: Rbfox3).

(D–I) Profile plots depicting the enrichment of H3K27ac (D–F) and H3K4me1 (G–I) at the aNPC (D and G), eA (E and H), and lA (F and I) unique H3K27ac peaks.

(J) Unsupervised clustering-based heatmap depicting the ChIP-seq enrichment of H3K4me1 during all stages of astrogliogenesis and H3K27ac during all stages of astrogliogenesis and neurogenesis at the H3K4me1 peaks in aNPC.

(K and L) Line plot depicting the enrichment of H3K4me1 during all stages of astrogliogenesis and H3K27ac during all stages of astrogliogenesis and neurogenesis in the regulatory regions of neuronal marker genes (K) and astroglial marker genes (L) that belong to clusters 2 and 6 or 7 from (J), respectively.

(M and N) H3K4me1 enrichment during neuronal differentiation at cluster 6 (M) and cluster 7 (N) sites.