REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Name	Company	Catalog #
Rabbit polyclonal AQP4	Santa Cruz Biotechnology	sc-20812; RRID:AB_2274338
Rabbit polyclonal ATF3 (C-19)	Santa Cruz	sc-188; RRID:AB_2258513
Rabbit polyclonal ATF3 (EPR19488)	Abcam	ab207434; RRID:AB_2734728
Mouse monoclonal beta III-tubulin	Sigma	T8660; RRID:AB_477590
Rat monoclonal CD44	BD PharMingen	550538; RRID:AB_393732
Rabbit polyclonal Connexin 43	Abcam	ab11370; RRID:AB_297976
Guinea pig polyclonal DCX	Millipore	AB2253; RRID:AB_1586992
Mouse monoclonal FLAG (M2 clone)	Sigma	F1804; RRID:AB_262044
Mouse polyclonal GAPDH	Abcam	ab9484; RRID:AB_307274
Mouse monoclonal GFAP	Sigma	G3893; RRID:AB_477010
Rabbit polyclonal GFAP	Dako	Z0334(29-2); RRID:AB_10013382
Rabbit polyclonal GLAST	Frontier Institute	AB_2571715; RRID:AB_2571715
Rabbit polyclonal H3K4me1	Abcam	ab8895; RRID:AB_306847
Rabbit polyclonal H3K27ac	Abcam	ab4729; RRID:AB_2118291
Rat Monoclonal ASCL1	R&D Systems	MAB2567; RRID:AB_2059503
Mouse polyclonal MYC	Cell Signaling	2276; RRID:AB_331783
Rabbit polyclonal NESTIN	BioLegend	839801; RRID:AB_2565443
Mouse polyclonal NeuN	Millipore	MAB377; RRID:AB_2298772
Rabbit polyclonal Nf-1A (K-21)	Santa Cruz	sc-133816; RRID:AB_10611351
Rabbit polyclonal NFIA	Abcam	ab41851; RRID:AB_944419
Rabbit polyclonal OLIG2	Millipore	AB9610; RRID:AB_570666
Rabbit polyclonal RFP	Biomol	600401379S; RRID:AB_11182807
Rabbit polyclonal Runx2 (M-70) X	Santa Cruz	sc-10758X; RRID:AB_2184247
Rabbit polyclonal S100	Abcam	ab868; RRID:AB_306716
Mouse polyclonal S100-beta	Abcam	ab11178; RRID:AB_297817
Rabbit polyclonal SATB2	Abcam	ab34735; RRID:AB_2301417
Rabbit polyclonal SOX9	Abcam	ab185966; RRID:AB_2728660
HRP anti-Goat IgG	Santa Cruz	SC-2020; RRID:AB_631728
Alexa Fluor 488 goat anti-mouse IgG	Invitrogen	A11001; RRID:AB_2534069
Alexa Fluor 488 donkey anti-mouse IgG	Invitrogen	A21202; RRID:AB_141607
Alexa Fluor 568 goat anti-mouse IgG	Invitrogen	A11004; RRID:AB_141371
Alexa Fluor 633 goat anti-mouse IgG	Invitrogen	A21050; RRID:AB_141431
Alexa Fluor 647 donkey anti-mouse IgG	Invitrogen	A31571; RRID:AB_162542
HRP anti-mouse IgG	Santa Cruz	SC-2005; RRID:AB_631736
Alexa Fluor 488 goat anti-rabbit IgG	Invitrogen	A11008; RRID:AB_143165
Alexa Fluor 488 donkey anti-rabbit IgG	Invitrogen	A21206; RRID:AB_141708
Alexa Fluor 568 goat anti-rabbit IgG	Invitrogen	A11011; RRID:AB_143157
Donkey IgG anti-Rabbit IgG (H+L)-Cy3	Dianova	711-165-152; RRID:AB_2307443
Alexa Fluror 633 goat anti-rabbit IgG	Invitrogen	A21070; RRID:AB_2535731
Alexa Fluor 647 donkey anti-rabbit IgG	Invitrogen	A31573; RRID:AB_2536183
HRP anti-rabbit IgG	Santa Cruz	SC-2004; RRID:AB_631746
Alexa Fluor 633 goat anti-rat IgG	Invitrogen	A21094; RRID:AB_141553
DAPI	Sigma	D9542; Sigma CAS# 28718-90-03
Bacterial and Virus Strains
DH5α Competent Cells	Invitrogen	18265017
One Shot TOP10 Chemically Competent E. coli	Invitrogen	C404010
Chemicals, Peptides, and Recombinant Proteins
1 Kb ladder	NEB	N3232S
100 bp ladder	NEB	N3231S
100x Glutamax	Invitrogen	35050-0380
10x Antractic Phos run Buffer	NEB	B0389 S
1-Bromo-3-Chloropropane	Sigma	B9673
1M Hydrochloric acid	Applichem	A1434.1000
2-Mercaptoethanol	Sigma	M3148-100ML
2-Propanol	Sigma	I9516-500ML
Acrylamide	AppliChem	A 1672
Agar	Merck	1119251000
Agarose	Lonza	98200-100
Ammonium persulfat	Amresco	M133-100 g
Ampicillin	Sigma	A5354-10 ml
Ampicillin Sodium Salt	AppliChem	4G017739
Annexin V	BD Biosciences	559934
Antrarctic Phosphase	NEB	M0289S
Aqua-poly mount	Polysciences	18606-20
B27 supplement	Life Technologies	17504-044
BioRad protein assay	GIBCO	500-0006
BrdU	Sigma	B5002-100MG
Bromphenol-blue	Merck	1.08112.0025
BSA(Cell culture)	Sigma	A9418-50 g
Chloroform	Sigma	C2432
Chloroform: Isoamyl alcohol 24:1	Sigma	C0549-1PT
Deoxycholic Acid Sodium salt	Amresco	0613-100G
DEPC water	Roth	K028.2
Dimethylsulfoxide	Sigma	D8418
DMEM	GIBCO	21969-035
DMEM-F12	GIBCO	21331-020
DNase I, RNase free	1000U	EN0525
Donkey serum	Merck Milipore	S30-100ml
dNTP mix	NEB	N0447S
Baytril (Enrofloxacin)	Baytril Bayer
EGF	PeproTech	AF-100-15-1 mg
EGTA	Sigma	E0396
Ethanol absolute	Sigma	34923
Ethidium bromide	Sigma	46065
Ethylene glycol	Sigma	324558
Ethylenediamine tetraacetic acid Disodiumsalt (EDTA)	Calbiochem	15576-028/324503
FBS	Invitrogen	10270-106
FGF-2	PeproTech	100-18B-100 ug
Formaldehyde	Sigma	F8775-500ML
Gelatin	Sigma	G1890-100 g
Glucose	Sigma	G8270
Glycerol 99%	Sigma	G5516-500ML
Glycine	Merck	8.16013.1000
Glycogen	Roche	10901393001
HEPES acid free	Amresco	0511-250G
HEPES potassium salt	Sigma	H0527
Isoflurane	Isova vet, Centauro	240055
Kanamycin sulfate	Sigma	60615-5 g
L-Glutamax	Invitrogen	35050-0380
LIF	N/A	Self-made
Lipofectamine 2000	Invitrogen	11668-019
Lipofectamine RNAiMAx	Invitrogen	13778-0150
Lithium chloride	VWR	437032G
Magnesium chloride anhydrous	Sigma	M8266
Meloxicam	VITA Laboratories	N/A
Methanol	Sigma	34860-2.5L-R
Milkpowder (skim)	Merck	1.15363.0500
N,N,N’,N’, Tetramethylethylenediamine	Sigma	T9281-50ML
N2 supplement	Life Technologies	17502-048
Natural mouse laminin	Invitrogen	23017-015
Neurobasal medium	GIBCO	21103-049
N-Lauroylsarcosine sodium salt	Sigma	L9150
Non-essential amino acids	GIBCO	1140-035
Nonidet P 40 substitute	Sigma	74385-1L
Oregon Green 488 BAPTA	Life Technologies	O6807
Opti-MEM	GIBCO	31985-047
Paraformaldehyde	Merck	8.18715.1000
PBS pH 7.2 (10x)	500 ml	70013-016
Pen/Strep, 10000u/ml	Invitrogen	15140122
Phenol solution	Sigma	P4557-100 ml
Phenylmethanesulfonyl fluoride	Amresco	0754-5G
Poly-l-ornithine hydrobromide	BD Biosciences	P3655-100 mg
Ponceau-solution	Sigma	P7170-1L
Potassium hydroxide, granular	Merck	1050330500
Propidium iodide	Sigma	P4170-10 mg
Protease inhibitor mix 100x	GE healthcare	80-6501-23
Protein A Agarose beads	Millipore	16-156
Protein G Agarose beads	Millipore	16-266
Proteinase K	Sigma	P2308-25mG
Purple loading dye	NEB	B7024 S
Quick Ligation kit	NEB	M2200S
Rbcl2	Sigma	83980-10 g
Rimadyl Carprofen	Pfizer	N/A
RNase	Applichem	A3832,0050
RNase away	Sigma	83931
Rneasy MiniElute spin columns	QIAGEN	1026497
S2-0.5% Trypsin-EDTA	GIBCO	15400-054
Saponin	VWR	440914Y
SDS-20%	Merck	428018-200ML
SeaBlue Protein Standard	Life Sciences	LC5925
di-Sodium tetraborate Decahydrat	Merck	1.01964.0025
Sodium azide	Sigma	S2202
Sodium bicarbonate	Applichem	A0384
Sodium chloride	Amresco	0241-1KG
Sodium fluoride	Merck	1.06449.0250
Sodium hydroxide	VWR	28244295
Sodium orthovanadate	Alfa Aesor	E11W016
Sodium Pyruvate	Sigma	P2256
Sybr Green	Invitrogen	4367659
T4 DNA Ligase	NEB	M0202S
T4 DNA Ligase Buffer	NEB	B0202S
T4 DNA polymerase	NEB	M0203S
Taq DNA Polymerase	NEB	M0267S
tert.-Butanol	Sigma	308250-1L
Thermo Pol Buffer	NEB	B9004S
Tri reagent	Sigma	T9424-100 ml
Tris Ultrapure	Invitrogen	15504-020
Tris-Hydrochloride	CalBiochem	648317
Triton x 100	Sigma	X100-1L
tRNA	Sigma	R5636-1 ml
Trypan blue	GIBCO	15250-061
Trypsin-EDTA, 0.5%	Life Technologies	15400054
Trypton Soy Agar	Merck	1.05458.0500
Tryptone	Sigma (Fluka)	T9410-250 g
Tween 20	Sigma	P1379-500ML
Vectashield mounting media	Vector	H-1000
Critical Commercial Assays
High Capacity cDNA RT kit	Invitrogen	4368814
Nextera DNA Library Prep Kit	Illumina	FC-121-1030
Power Sybr Green PCR Master Mix	Life Technologies	4367659
Deposited Data
RNA-seq during astrogliogenesis and upon TF depletion	This study	GEO: GSE96539
H3K27ac ChIP-seq during astrogliogenesis and upon TF depletion	This study	GEO: GSE96539
H3K4me1 ChIP-seq during astrogliogenesis	This study	GEO: GSE96539
Experimental Models: Cell lines
ESCs	ATCC	SCRC-1002; RRID:CVCL_5746
Experimental Models: Organisms/Strains
C57BL/6J	The Jackson Laboratory	Jax#000664; RRID:IMSR_JAX:000664
Oligonucleotides
See Table S7 for primer sequences	This study	N/A
See Table S7 for siRNAs sequences	Dharmacon	N/A
Recombinant DNA
pCIDR-dsRed	Pataskar et al., 2016	N/A
pCIDR-Nfia-dsRed	This Study	N/A
pCIDR-Atf3-dsRed	This Study	N/A
pCIDR-Runx2-dsRed	This Study	N/A
Software and Algorithms
ImageJ	NIH	N/A; RRID:SCR_003070
ZEN	Carl Zeiss Microscopy	N/A; RRID:SCR_013672
FACSCanto II using DIVA software	BD Biosciences	N/A; RRID:SCR_001456
HCImage software	Hamamatsu Corporation	N/A; RRID:SCR_015041
Prism	Graphpad	N/A; RRID:SCR_005375
TopHat v.2.0.8	JHU (Trapnell et al., 2009)	N/A; RRID:SCR_013035
SAMTOOLS v0.1.19	University of Birmingham (Li et al., 2009)	N/A; RRID:SCR_002105
HTSeq program v0.5.4p1	Python package (Anders et al., 2015)	N/A; RRID:SCR_005514
DESeq	Bioconductor (Oshlack et al., 2010)	N/A; RRID:SCR_000154
ToppGene	Cincinnati Children’s Hospital Medical Center (Chen et al., 2009)	N/A; RRID:SCR_005726
pheatmap R package	R package	N/A; RRID:SCR_016418
Bowtie v0.12.9	JHU (Langmead, 2010)	N/A; RRID:SCR_016368
QuasR package	Bioconductor (Gaidatzis et al., 2015)	N/A; RRID:SCR_006820
MACS v2.0.10.20120913	Liu’s Lab (Zhang et al., 2008)	N/A; RRID:SCR_013291
ngs.plot.r program	(Shen et al., 2014)	N/A; RRID:SCR_011795
HOMER v4.7	HOMER	N/A; RRID:SCR_010881
findMotifs.pl program	HOMER	N/A; RRID:SCR_016417