Figure 2.

Co-operative Activation of CAR19-iNKT Cells

(A) Dot plots showing expression of CD19 and CD1d in parental and derivative K562 cells after retroviral transduction of corresponding cDNAs.

(B) Cytotoxic activity of second-generation CAR19-iNKT cells against parental CD1d^–19^– K562 cells (WT) or K562 cells expressing CD1d and CD19 singly or in combination at the indicated E/T cell ratios (representative of three experiments).

(C) Cytotoxic activity of second-generation CAR19-iNKT cells against parental CD19^–CD1d^– K562 cells or K562 cells expressing CD19 and CD1d singly or in combination with (right) and without (left) pre-pulsing of targets with 100 ng/mL αGalCer. E/T cell ratios are as shown (representative of two experiments).

Error bars represent SEM of triplicate assays.