Fig. 2. Downregulation of BAP1 promotes proliferation, cell cycle progression, and invasion of ICC in vitro and ICC progression in vivo.

a The mRNA expression and protein expression of BAP1 in ICC cell lines (HCCC9810, RBE, HCCC9810-Mock, HCCC9810-BAP1, RBE-Mock, and RBE-shBAP1) were shown. b Effects of BAP1 overexpression and downregulation on proliferation using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. c Effects of BAP1 overexpression and downregulation on cell cycle progression using flow cytometry after propidium iodide staining. Representative images were shown. d Effects of BAP1 overexpression and downregulation on migration using scratch wound healing assay. Representative images were shown. Scale bars = 200 μm. e Effects of BAP1 overexpression and downregulation on invasion using Matrigel invasion assay. Representative images were shown. Scale bars = 100 μm. f Effects of BAP1 overexpression and downregulation on the growth of in vivo subcutaneous xenograft tumors. Tumor volume and weight of xenografts derived from HCCC9810-BAP1 cells were significantly reduced as compared with those of tumors derived from HCCC9810-Mock cells (n = 5); tumor volume and weight of xenografts derived from RBE-shBAP1 cells were markedly increased as compared with those of tumors derived from RBE-Mock cells (n = 5). All bar graphs depicted quantification of triplicate results with mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001