Fig. 2.

Lrh1^IEC-KO mouse organoids have diminished Notch activation. a Representative immunoblot for cleaved Notch1 in Lrh1^fl/fl (left two lanes) and Lrh1^IEC-KO (right two lanes), n = 4 per condition. b Fold change in expression by RT-qPCR in Lrh1^fl/fl (black) and Lrh1^IEC-KO (gray) organoids for Notch1 and the Notch target genes Olfm4 and Hes1. Relative expression of the stem cell marker Lgr5, Paneth cell marker Lysozyme, and goblet cell marker Mucin2 are also shown. Minimum of three replicates per condition. c Histology of Lrh1^fl/fl and Lrh1^IEC-KO small intestine showing goblet (DBA, red, left), Paneth (lysozyme, green, middle) and enterochromaffin cells (5HT, red, right and inset). Scale bar = 50 μm. Intestinal crypts are outlined with dashed white line in middle panel. d Quantitation of epithelial subtype distribution, n = 3 animals per condition. For b and d, error bars are SEM using Student’s t test (unpaired, two tailed) with p values of *p = < 0.05, **p = < 0.01, ***p = < 0.001, and ****p = < 0.0001