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. 2018 Oct 10;8:15057. doi: 10.1038/s41598-018-32888-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal-specific binding of the β-catenin complex to the loci of target genes of WNT/β-catenin signaling. (A) Quantitative RT-PCR analysis of gene expression of the indicated genes in C2C12 cells treated with vehicle (control) or IWR1-endo in the proliferation and muscle differentiation stages (n = 3 each group). **p < 0.005, ***p < 0.001. (B) ChIP-qPCR analysis of β-catenin binding on the promoter regions of the indicated genes using β-catenin antibody. ChIP with IgG at proliferation (Pro) stage (green), α-β-catenin antibody at proliferation stage (orange), IgG at myogenic differentiation (Diff) stage (blue), and α-β-catenin antibody at myogenic differentiation stage (red) (n = 3 each group). ***p < 0.001. NS, not significant.