Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 10;8:15057. doi: 10.1038/s41598-018-32888-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HATs and β-catenin coordinately regulate cell proliferation and differentiation through the regulation of Ccna2, Cdc25c and Fermt2. (A) Quantitative RT-PCR analysis of indicated genes after siRNA knockdown of control (blue) or Kat2b (red) (n = 3 each group). ***p < 0.001. (B) Quantitative RT-PCR analysis of indicated genes after siRNA knockdown of control (blue) or Cbp (red) (n = 3 each group). **p < 0.01, ***p < 0.001. (C) ChIP-qPCR analysis of H3K9Ac on the Ccna2 and Cdc25c promoter regions with Kat2b siRNA knockdown for 24 hours at the proliferation stage. ChIP with IgG with control siRNA (green), IgG with Kat2b siRNA knockdown (blue), α-H3K9Ac antibody with control siRNA (orange), and α-H3K9Ac antibody with Kat2b siRNA knockdown (red) (n = 3 each group). ***p < 0.001. (D) ChIP-qPCR analysis of H3K27Ac on the Fermt2 promoter region after Cbp siRNA knockdown for 24 hours at the proliferation stage. ChIP with IgG with control siRNA (green), IgG with Cbp siRNA knockdown (blue), α-H3K27Ac antibody with control siRNA (orange), and α-H3K27Ac antibody with Cbp siRNA knockdown (red) (n = 3 each group). ***p < 0.001. (E) ChIP-qPCR analysis of β-catenin binding on the Ccna2 and Cdc25c promoter regions following Kat2b siRNA knockdown for 24 hours at the proliferation stage. ChIP with IgG with control siRNA (green), IgG with Kat2b siRNA knockdown (blue), α-β-catenin antibody with control siRNA (orange), and α-β-catenin antibody with Kat2b siRNA knockdown (red) (n = 3 each group). **p < 0.01, ***p < 0.001. (F) ChIP-qPCR analysis of β-catenin binding on the Fermt2 promoter region following Cbp siRNA knockdown for 24 hours at the proliferation stage. ChIP with IgG with control siRNA (green), IgG with Cbp siRNA knockdown (blue), α-β-catenin antibody with control siRNA (orange), and α-β-catenin antibody with Cbp siRNA knockdown (red) (n = 3 each group). ***p < 0.001. (G) BrdU staining in C2C12 cells after control or Kat2b siRNA knockdown (KD) for 24 h. Nuclei are counterstained with hematoxylin. Arrows indicate BrdU-positive cells. Scale bar, 50 µm. Graph shows the quantification of BrdU-positive cells. ***p < 0.001. (H) Immunocytochemical analysis of MYH (green) in differentiated C2C12 cells with control or Cbp siRNA knockdown (KD). Scale bar, 50 µm. Graph shows the quantification of the length of myotubes (middle) and fusion index (right). ***p < 0.001.