Fig. 2.

(a) Distribution of the length of the scars left by recombination events that resulted in removing the inserted genes. The vertical red lines indicate the exact position in which the genes were inserted (5ʹ and 3ʹ, respectively). The figure illustrates how deletions in some cases included sequences outside the insert. (b) Distribution of length of the scars for the different insertion sites. TEV-2NIb1 is inserted at cloning site 1, TEV-AlkB at cloning site 1.5, and TEV-2NIb2, TEV-2HC-Pro, TEV-2NIa-Pro, and TEV-eGFP at cloning site 2. In all cases, the dark line represents the mean, boxes represent the interquartile range, the whiskers extend to the most extreme data point, which is no more than range times the interquartile range, and widths are proportional to the square-roots of the number of observations in the group