Fig. 4.

Validation of RNA-seq by qPCR. a Approximate locations of predicted transcription motifs on Dcn and Col3a1 (MatInspector). b Wiggle plots of Dcn and Col3a1 from RNA-seq. Each respective gene is illustrated below its expression track (blue). Red line indicates the greatest number of reads for each track. c Dcn, Col3a1, and Henmt1 mRNA expression in OFC. Data are presented as fold change of mean values in the saline group. Error bars indicate SEM. *p < 0.05; n = 4 per group