Figure 2.

Liver sinusoidal endothelial cells up-regulate expression of F4/80 and CX₃CR1 in splenic Ly6C⁺ monocytes. (a) Isolated F4/80^lowCD11b⁺ monocytes and F4/80^highCD11b⁺ macrophages of spleen were subjected to Giemsa staining. Bar = 10 μm. (b,c) Splenic F4/80^lowLy6C⁺ monocytes were co-cultured with LSECs of vehicle or D2 clodronate-treated mice for 24 h. They were subjected to flow cytometry and Giemsa staining. Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01 compared to 0 h. The results represent three independent experiments.