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. 2018 Oct 10;8:15076. doi: 10.1038/s41598-018-33440-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CX₃CR1 deficiency attenuates alcoholic liver injury. CX₃CR1^+/GFP (n = 4) and CX₃CR1^GFP/GFP (n = 4) mice were fed 5% liquid ethanol diet for 8 weeks. (a,b) Body weight changes and serum levels of ALT, AST, and triglyceride were measured. (c) Liver sections were stained with hematoxylin and eosin (H & E) and Oil-Red O, or with F4/80 antibody. Bar = 100 μm. (d) Isolated liver MNCs were subjected to flow cytometry. (e) Isolated F4/80^high cells were subjected to qRT-PCR analysis. Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01 compared to corresponding controls. The results represent two independent experiments.