Fig. 5.

Myocardin-dependent micro-RNA, miR-133a, regulates mitochondrial function and permeability transition. a–c H9c2 cells were transfected with miR-133a or an empty vector, and treated with FSK-I (18 h), or DMSO as a control vehicle; stained with TMRM (a) or (b) with calcein-AM and CoCl₂, or (c) with calcein-AM and Eth HD-1. d H9c2 cells were transfected with a SR-targeted calcium biosensor, treated with FSK-I, and quantified. e H9c2 were transfected with miR-133a or an empty vector, a mito-targeted calcium biosensor, treated with FSK-I, and quantified. f H9c2 cells were transfected with a miR-133a mimic, and treated with FSK-I or vehicle control. Cells were injected with oligomycin (1 µM) (a), FCCP (1 µM) (b), and antimycin A (1 µM) and rotenone (1 µM) (c). OCR was corrected by total cell number following analysis (n = 4). g Calculated respiration rates from f. h H9c2 cells were transfected with sh-Myocd or scrambled control, miR-133a, a mito-targeted calcium biosensor, and quantified. i H9c2 cells were transfected with miR-133a or an empty vector control, Nix and a mito-targeted calcium biosensor. Cells were treated with FSK-I (18 h), or DMSO as a control vehicle and quantified. Data are expressed as mean ± SE. *p < 0.05 compared to control, **p < 0.05 compared to treatment, determined by one-way ANOVA