Fig. 4. T-96 inhibits the expression of genes related to cell cycle progression and DNA replication.

a, b GO analysis of genes downregulated (determined via whole transcriptome analyses (RNA-seq)) in LN-229 or U-87 cells after treament with T-96. The top 10 biological process terms based on fold enrichment are shown. c, d Real-time quantitative PCR analyses of cell cycle-related and DNA replication-related genes in LN-229 and U-87 cells after treatment with or without 10 μM T-96 for the indicated time. e Western blot analyses of several components of the replication complex and associated regulators, including ORC6, CDC6, CDT1, MCMs and CDC45. Tubulin was used as the control