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. 2018 Oct 10;8:15063. doi: 10.1038/s41598-018-33150-2

Figure 4.

Figure 4

Pharmacological inhibition of GR attenuates glucocorticoid-induced protein expression of LEDGF/p75 and CLU in 22Rv1 cells. 22Rv1 and PC3 cells were co-treated with 10 nM Dex and 100 nM Mif for up to 48 hours. Untreated cells were used as controls. Whole cell lysates were prepared for Western blotting analysis and probed with anti-GR, anti-LEDGF/p75, and anti-sCLU antibodies. Unpaired t-test statistical analysis revealed that Mif attenuated LEDGF/p75 and CLU expression in 22Rv1 cells. (A) However, this attenuation was not observed in PC3 cells. (B) (*p < 0.05) All blots represented within panels, although cropped, are derived from CLU or LEDGF/p75 and loading control images from the same gel. Full-length blots/gels are presented in Supplementary Figure S2.