Fig. 4.

ZV E protein modulates cell cycle dynamics of fNSCs and induces expression of pro-neural genes. a, b Representative images of DNA content analysis by propidium iodide (PI) staining using FACS 24 h post transfection of either empty vector or E protein in human fNSCs. c Bar graph shows quantitative analysis of data obtained from PI staining depicting percentage of cells in G0/G1, S phase and G2/M phase of cell cycle in indicated groups. d Bar graph represents relative mRNA levels of pro-neural genes (PTN, ROBO2, SPOCK1, and DCX) normalized with 18S mRNA levels, 24 h post transfection of empty vector and E protein in indicated groups. The values represent the mean ± standard error of three independent experiments (n = 3); *p < 0.05