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. 2018 Oct 10;50(10):132. doi: 10.1038/s12276-018-0160-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a SDS-PAGE/Coomassie blue staining and immunoblot analyses with the indicated antibodies for purified FLAG-MSK1 from HEK293T cells in the absence (MSK1, lane 1) and presence (MSK1*, lane 2) of MKK6ca co-expression. b Effects of MSK1 in p53-mediated in vitro transcription of a chromatin template. Standard p53- and p300-dependent transcription from a chromatinized p53ML plasmid was performed according to the scheme in the top panel. Reactions contained 5 ng (lanes 5 and 7) or 20 ng (lanes 6 and 8) of MSK1 where indicated. c Direct effects of histone H3 phosphorylation in the p53-mediated in vitro transcription of a chromatin template. In vitro transcription assays were performed with chromatin templates assembled with recombinant histone octamers containing fully phosphorylated H3S10 or H3S28. Relative transcription levels were measured using a phosphoimager and normalized to that of p53 and p300 addition (b and c, lane 4)