Figure 1.

(A) vasopressin stimulation profile used during the course of the experiment; (B) Left: 2D cross section of an idealized liver lobule with locations of portal triads and central vein. Blood flows from the periportal region toward the pericentral region. Right: Manually segmented hepatocytes with approximate locations of central veins and portal triads in the imaged area. Different colors represent different manually segmented hepatocytes. The hepatocytes are distributed across different liver lobules; (C) heat map of cytosolic calcium intensities across all 1,300 segmented hepatocytes over 1,600 s. The data suggests an overall synchronization of calcium spikes across the imaged area; (D) Sequential cytosolic Ca²⁺ spiking in 10 hepatocytes residing in the region between a central vein and a portal triad. All hepatocytes are in the region within the white box in (B). Propagating Ca²⁺ waves interspersed with asynchronous spiking can be seen; (E) Ca²⁺ wave propagating away from a central vein.