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. 2018 Oct 4;9:2297. doi: 10.3389/fimmu.2018.02297

Figure 3.

Figure 3

TLR4 knockdown and BAY 11-7082 reduce the AFB1-promoted SIV replication and inflammatory responses. PAMs were infected with SIV (MOI = 1) and then treated with (AFB1 group) or without (control group) 0.05 μg/ml AFB1. A TLR4-specific siRNA sequence was used to remove the effects of TLR4, and a control siRNA sequence was used as a negative control. (A) The knockdown efficiency of TLR4 siRNA in PAMs. (B) Viral titers and (C) relative viral M mRNA levels, (D) NP, (E) pp65 and (F) TNF-α mRNA levels. A specific inhibitor of NFκB, BAY 11-7082 (10 μM), was added to remove the effects of NFκB, and medium was used as a negative control. (G) Viral titers, (H) relative viral M mRNA levels, and (I) TNF-α mRNA levels. Data are presented as the means ± SEM of three independent experiments. Significance compared with the control group, *P < 0.05, **P < 0.01; significance compared with the negative control group, #P < 0.05 and ##P < 0.01.