Table 1.

Primers sequences for real-time PCR.

Source	Gene	Forward (5′-3′)	Reverse (3′-5′)
Virus	M	GGGAAGAACACCGATCTTGA	CTCCGTTCCCATTAAGAGCA
Pig	TLR1	GAAACTACAAGGGCAGCTGG	GGGAAACTGAACACCTCCCT
	TLR2	AGACGCTGGAGGTGTTGG	AACGAAGCATCTGGGAGT
	TLR3	AAAACCAGCAACACGACT	TTGGAAAGCCCATAAAGA
	TLR4	AGAATGAGGACTGGGTGA	TGTAGTGAAGGCAGAGGT
	TLR5	GGCTCAACCAAACCAACG	GGGTGATGACGAGGAATAG
	TLR6	AACTCACCAGAGGTCCAA	TCTTCCCTGTCGATTCTC
	TLR7	GGCAAGTAGAGGACAT	GGTAGACCCTGAACAT
	TLR8	CGGCACCAGAAGAACG	GGCAGGTCAGGAGCAA
	TLR9	GGCCTTCAGCTTCACCTTGG	GGTCAGCGGCACAAACTGAG
	TLR10	ATGATTCGGCCTGGGTAAAG	TTGCCAGGATCAGAGTTTCC
	IL-10	CTGCCTCCCACTTTCTCTTG	TCAAAGGGGCTCCCTAGTTT
Mouse	TNF-α	GACTCAGATCATCGTCTC	GGAGTAGATGAGGTACAG
	GAPDH	CCACCCAGAAGACTGTGGAT	AAGCAGGGATGATGTTCTGG
	TLR4	CACTGTTCTTCTCCTGCCTGAC	CCTGGGGAAAAACTCTGGATA
	18S	TTGACGGAAGGGCACCACCAG	GCACCACCACCCACGGAATCG

M, influenza A virus matrix protein; TLR, toll-like receptor; IL-10, interleukin 10; TNF-α, tumor necrosis factor-α; GAPDH, glyceraldehyde-phosphate dehydrogenase.