TABLE 4.
Biolog Phenotype MicroArray comparisons for WT strain CL150 compared to the all-ECF σ deletion strain RFF625ca
| Plate, wellb | Substratec | AUC of CL150/AUC of RFF625cd |
P value | Substrate descriptione |
|---|---|---|---|---|
| PM09, H06 | Sodium nitrate (100 mM) | 1.52 | 7.4E−08 | Osmolyte |
| PM15, D06 | Domiphen bromide no. 2 | 1.81 | 4.3E−07 | Quaternary ammonium compound |
| PM19, D05 | Iodonitrotetrazolium violet no. 1 |
1.44 | 7.4E−06 | Tetrazolium dye |
| PM02, C04 | d-Melezitose | 1.56 | 1.2E−05 | Carbon source, trisaccharide |
| PM20, H07 | Tolylfluanid no. 3 | 0.43 | 3.7E−05 | Antibacterial, phenylsulfamide |
| PM12, B12 | Polymyxin B no. 4 | 1.70 | 8.9E−05 | Antibacterial, cationic peptide-fatty acid |
| PM18, F08 | Tinidazole no. 4 | 1.89 | 9.1E−05 | Antibacterial, nitroimidazole |
| PM19, C04 | Chlorhexidine no. 4 | 0.59 | 1.0E−04 | Disinfectant, cationic bisbiguanide |
| PM12, E10 | Benzethonium chloride no. 2 |
1.57 | 1.2E−04 | Quaternary ammonium compound |
| PM04, E05 | O-Phosphoryl-ethanolamine | 0.58 | 3.3E−04 | Phosphorous source |
| PM11, H12 | Ofloxacin no. 4 | 1.48 | 4.1E−04 | Antibacterial, fluoroquinolone |
| PM13, B04 | Azlocillin no. 4 | 1.57 | 4.7E−04 | Antibacterial, penicillin |
| PM11, D02 | Capreomycin no. 2 | 1.69 | 1.3E−03 | Antibacterial, cyclic peptide |
| PM11, E08 | Enoxacin no. 4 | 1.95 | 2.1E−03 | Antibacterial, fluoroquinolone |
| PM09, E08 | Urea (3%) | 1.45 | 5.2E−03 | Osmolyte |
| PM13, G06 | Manganese(II) chloride no. 2 |
1.30 | 5.5E−03 | Heavy metal |
| PM11, B12 | Lomefloxacin no. 4 | 1.40 | 6.2E−03 | Antibacterial, fluoroquinolone |
| PM11, C03 | Bleomycin no. 3 | 1.65 | 9.0E−03 | Antibacterial, peptide- polyketide |
| PM19, A01 | Josamycin no. 1 | 1.26 | 9.6E−03 | Antibacterial, macrolide |
| PM04, A02 | Sodium phosphate | 1.35 | 1.0E−02 | Phosphorous source |
| PM20, B09 | Tetrazolium violet no. 1 | 1.40 | 2.4E−02 | Tetrazolium dye |
| PM04, H11 | Methane sulfonic acid | 0.76 | 4.2E−02 | Sulfur source |
| PM15, B06 | EDTA no. 2 | 1.79 | 4.6E−02 | Chelating agent |
Biolog Phenotype MicroArray comparisons were identified using the opm package (63 [see Materials and Methods]) for WT CL150 compared to the all-ECF σ deletion strain RFF625c.
Shown are the plate number and then well number of Biolog Phenotype MicroArray 96-well plates. PM2, carbon sources; PM4, phosphorous and sulfur sources; PM9, osmolytes; PM11 to -20, chemical sensitivity tests for bacteria.
For chemical stress tests, the number indicates which of the four concentrations (where 1 is lowest and 4 is highest) had a significant effect.
AUC, area under the concentration-time curve (see Materials and Methods).
Compounds listed as "antibacterial" possess antibacterial activity; however, their primary commercial use may not be treatment of bacterial infections (examples include tolylfluanid, tinidazole, and bleomycin).