FIG 1.
(A) Amino acid sequences for full-length and mature rNg-ACP proteins. The sequences highlighted in red are encoded by the vector, the turquoise sequence is the 6×His tag for purification by affinity chromatography, and the green sequence is the leader peptide sequence. (B) SDS-PAGE analysis of full-length and mature rNg-ACP proteins. Single bands with purity of >95% are shown for full-length (Mr = ∼18,000) and mature (Mr = ∼12,000) rNg-ACP proteins. (C) SEC chromatogram of purified mature rNg-ACP, showing a single major peak of protein for crystallography (Mr = ∼12,000).