FIG 10.

Antibodies to rNg-ACP prevent rNg-ACP from inhibiting HL lytic activity on M. lysodeikticus cells. (A) HL inhibitory activity by rNg-ACP (0.5 µg/ml) was analyzed as a reduction in ODλ₅₉₅ nm (Abs, absorbance) against time for an M. lysodeikticus cell suspension (1 mg/ml) in the presence or absence of decomplemented rabbit anti-rNg-ACP sera (Terminal Bleed [TB]) and sera from the same animal prior to immunization (preimmunization bleed [PB]). The symbols represent the mean absorbance (ODλ₅₉₅ nm) (from n = 3 independent experiments), and the error bars represent the corresponding standard errors of the means (SEM). (B) Determination of percentage of M. lysodeikticus cell lysis for each test condition presented in panel A for the time point of 2 h of incubation only. The columns represent mean percent lysis data (from n = 3 independent experiments), and the error bars represent the corresponding SEM.