FIG 3.

Structure-based sequence alignment of Ng-ACP with Nm-ACP and the MliC/PliC family of lysozyme inhibitor proteins. The structure-based sequence alignment for N. meningitidis serogroup B strain MC58 (E6MZT7), St-PliC from S. enterica Typhimurium (Q8ZPY8), Pa-MliC from Pseudomonas aeruginosa (Q91574), and Ba-PliC from Brucella abortus (Q57ES7) described by Humbert et al. (15) was expanded with the addition of the sequence of Ng-ACP from Neisseria gonorrhoeae P9-17. β-Strands (including β-strand 1 to β-strand 8) are indicated by the shaded arrows and helix α1 by the shaded barrel; conserved cysteine residues of the mature proteins are blocked in blue and sequence motifs involved in the interaction of MliC (P. aeruginosa) with hen egg white lysozyme are indicated in red and green, as described previously (15). Amino acid residues highlighted in yellow show the differences between Ng-ACP and Nm-ACP type I sequences.