FIG 7.

Expression of Ng-ACP protein in the CDCP/FDA N. gonorrhoeae isolate panel. Bacterial lysates were reacted with rabbit anti-ACP serum in Western blots, and the level of Ng-ACP in each lysate was expressed as a ratio of the densitometry of band intensity compared to LPDA. Statistical differences in ratio values were analyzed for each isolate against P9-17 as a reference, which resulted in distribution of the isolates into three main groups, i.e., lower than P9-17 (P < 0.05) (blue columns), similar to P9-17 (P ≥ 0.05) (yellow columns), and higher than P9-17 (P < 0.05) (black columns) Ng-ACP levels. Columns represent means of results from a minimum of three independent measurements of densitometry, and the error bars represent the standard deviations.