TABLE 3.

Determination of Ng-ACP allele in isolates of the Neisseria gonorrhoeae CDCP/FDA collection^a

AR-Bank no.	GC no.	Sanger run accession no.	Sanger strain no.	Pubmlst/ Neisseria ID	Ng-ACP allele	Level of Ng-ACP protein expression relative to P9-17
165	GC-01	ERR854938	12CFX_T_043	37079	10	High
166	GC-02	ERR854921	12CFX_T_026	37062	10	Low
167	GC-03	ERR855352	12AZI_T_002	37507	10	Low
168	GC-04	ERR854924	12CFX_T_029	37065	10	Low
169	GC-05	ERR854922	12CFX_T_027	37063	10	Similar
170	GC-06	ERR854897	12CFX_T_001	37037	10	Low
171	GC-07	ERR854937	12CFX_T_042	37078	10	Low
172	GC-08	ERR854906	12CFX_T_010	37046	10	Low
173	GC-09	ERR854907	12CFX_T_011	37047	10	Similar
174	GC-10	ERR854900	12CFX_T_004	37040	10	High
175	GC-11	ERR855365	12AZI_T_015	37520	10	Low
176	GC-12	ERR854869	12CFX_T_045	37081	10	Low
177	GC-13	ERR855355	12AZI_T_005	37510	6	High
178	GC-14	ERR855325	12AZI_C_010	37480	10	Low
179	GC-15	ERR855356	12AZI_T_006	37511	6	High
180	GC-16	ERR854913	12CFX_T_017	37053	10	Similar
181	GC-17	ERR855357	12AZI_T_007	37512	6	High
182	GC-18	ERR854870	12CFX_T_047	37083	10	Low
183	GC-19	ERR854902	12CFX_T_006	37042	10	Low
184	GC-20	ERR854916	12CFX_T_020	37056	10	Similar
185	GC-21	ERR854898	12CFX_T_002	37038	10	Low
186	GC-22	ERR854919	12CFX_T_023	37059	10	Similar
187	GC-23	ERR855360	12AZI_T_010	37515	10	Low
188	GC-24	ERR854932	12CFX_T_037	37073	10	High
189	GC-25	ERR854903	12CFX_T_007	37043	10	Low
190	GC-26	ERR854936	12CFX_T_041	37077	10	Similar
191	GC-27	ERR854899	12CFX_T_003	37039	10	Low
192	GC-28	ERR854917	12CFX_T_021	37057	10	High
193	GC-29	ERR855351	12AZI_T_001	37506	10	Similar
194	GC-30	ERR854905	12CFX_T_009	37045	10	High
195	GC-31	ERR854928	12CFX_T_033	37069	10	High
196	GC-32	ERR854927	12CFX_T_032	37068	6	High
197	GC-33	ERR855353	12AZI_T_003	37508	6	High
198	GC-34	ERR854904	12CFX_T_008	37044	10	Similar
199	GC-35	ERR855358	12AZI_T_008	37513	10	Similar
200	GC-36	ERR854929	12CFX_T_034	37070	10	High
201	GC-37	ERR854912	12CFX_T_016	37052	10	Similar
202	GC-38	ERR855359	12AZI_T_009	37514	6	High
203	GC-39	ERR854908	12CFX_T_012	37048	10	Similar
204	GC-40	ERR854867	12CFX_T_024	37060	10	High
205	GC-41	ERR956689	12CFX_T_039	58867	10	High
206	GC-42	ERR956690	12CFX_T_030	58866	10	Low
207	GC-43	ERR855324	12AZI_C_009	37479	10	Low
208	GC-44	ERR854930	12CFX_T_035	37071	10	Similar
209	GC-45	ERR854933	12CFX_T_038	37074	10	Low
210	GC-46	ERR854911	12CFX_T_015	37051	10	Similar
211	GC-47	ERR854910	12CFX_T_014	37050	10	Similar
212	GC-48	ERR854939	12CFX_T_046	37082	10	Similar
213	GC-49	ERR854920	12CFX_T_025	37061	10	Low
214	GC-50	ERR854935	12CFX_T_040	37076	10	Low

^aThe sequence accession number (https://www.cdc.gov/drugresistance/resistance-bank/currently-available.html) for each isolate is linked to the SRA webpage (https://www.ncbi.nlm.nih.gov/sra/?term=SAMEA3165293) that provides the run accession number that can be used to search the Sanger repository (http://www.sanger.ac.uk/resources/downloads/bacteria/neisseria.html#project_3416) that can identify the strain information. The strain information is then used to search the pubmlst/Neisseria webpage (https://pubmlst.org/bigsdb?db=pubmlst_neisseria_isolates&page=query) to identify the ID number, amino acid sequence, and encoding allele. Statistical differences in ratio values were analyzed for each isolate against P9-17 as a reference, which resulted in distribution of the isolates into three main groups, i.e., lower than P9-17 (P < 0.05), similar to P9-17 (P ≥ 0.05), and higher than P9-17 (P < 0.05) Ng-ACP levels.