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. 2018 Oct 10;3(5):e00331-18. doi: 10.1128/mSphere.00331-18

TABLE 4.

ELISA reactivity of murine antisera raised to full-length and mature rNg-ACP proteins tested against immunizing protein and the densitometry arbitrary units for the corresponding anti-rNg-ACP Western blot bands shown in Fig. 9a

Immunogen Adjuvant Value × 103 of reciprocal
geometric mean ELISA titer
(95% confidence limits)
against immunizing protein
Densitometry arbitrary
values for Western
blot bands on P9-17
OM (Fig. 9)
Full-length rNg-ACP Saline 132 (76, 234) 1,835
Al(OH)3 4,230 (232, 77063) 2,438
Liposomes 18 (4, 84) 672
Liposomes + MPLA 1,993 (205, 19409) 2,280
ZW 3-14 micelles 14 (2, 81) 474
ZW 3-14 micelles + MPLA 164 (60, 449) 812
Mature rNg-ACP Saline 92 (1, 10,880) 99
Al(OH)3 13 (4, 40) 765
Liposomes 14 (1, 206) 659
Liposomes + MPLA 239 (1, 94,335) 3,014
ZW 3-14 micelles 7 (1, 37) 341
ZW 3-14 micelles + MPLA 16 (5, 46) 428
a

All mice were administered three 20-µg doses. Murine antisera were tested in solid-phase ELISA against the respective homologous immunizing recombinant proteins. Data represent the geometric means of the reciprocal ELISA titers of n = 5 animals per immunization group, with the 95% confidence limits indicated in parentheses. Serum from sham-immunized animals and normal mouse serum showed no reactivity in ELISA against the recombinant proteins (i.e., the absorbance at λ450 nm of antisera tested at a starting dilution of 1/100 was <0.1, i.e., similar to the level seen with no-serum background controls). The intensities of the individual Western blot bands on homologous P9-17 OM for antisera raised to the full-length and mature rNg-ACP proteins shown in Fig. 9 were calculated as arbitrary units of densitometry using ImageJ software.