TABLE 4.
Immunogen | Adjuvant | Value × 103 of reciprocal geometric mean ELISA titer (95% confidence limits) against immunizing protein |
Densitometry arbitrary values for Western blot bands on P9-17 OM (Fig. 9) |
---|---|---|---|
Full-length rNg-ACP | Saline | 132 (76, 234) | 1,835 |
Al(OH)3 | 4,230 (232, 77063) | 2,438 | |
Liposomes | 18 (4, 84) | 672 | |
Liposomes + MPLA | 1,993 (205, 19409) | 2,280 | |
ZW 3-14 micelles | 14 (2, 81) | 474 | |
ZW 3-14 micelles + MPLA | 164 (60, 449) | 812 | |
Mature rNg-ACP | Saline | 92 (1, 10,880) | 99 |
Al(OH)3 | 13 (4, 40) | 765 | |
Liposomes | 14 (1, 206) | 659 | |
Liposomes + MPLA | 239 (1, 94,335) | 3,014 | |
ZW 3-14 micelles | 7 (1, 37) | 341 | |
ZW 3-14 micelles + MPLA | 16 (5, 46) | 428 |
All mice were administered three 20-µg doses. Murine antisera were tested in solid-phase ELISA against the respective homologous immunizing recombinant proteins. Data represent the geometric means of the reciprocal ELISA titers of n = 5 animals per immunization group, with the 95% confidence limits indicated in parentheses. Serum from sham-immunized animals and normal mouse serum showed no reactivity in ELISA against the recombinant proteins (i.e., the absorbance at λ450 nm of antisera tested at a starting dilution of 1/100 was <0.1, i.e., similar to the level seen with no-serum background controls). The intensities of the individual Western blot bands on homologous P9-17 OM for antisera raised to the full-length and mature rNg-ACP proteins shown in Fig. 9 were calculated as arbitrary units of densitometry using ImageJ software.