Figure 2.

Formation of RER-Like Single Helicoidal Cisternae Stacks

(A) Schematic illustration of RER in cells.

(B and C) Typical fluorescence images of the right-handed (B) and left-handed (C) RER-like helicoids visualized in z -stacks by laser confocal microscopy. The yellow dash arrows indicate the midline of the cisternae.

(D) Schematic illustration of variation of the cisternae in z stacks under laser confocal fluorescence microscopy for cisternae stacks with different chirality. The red dash arrows indicate that there exist connections between two cisternae.

(E) The 3D reconstruction of the z stacks in (B) and (C) (middle) or the midline of the cisternae (right). The yellow dashed arrows indicate the spiral directions of the helicoids.

(F) Counted ratio of right- to left-handed stacks formed in solution with different Φ_ethanol.

Scale bars, 10 μm. Error bars are ±SEM. See also Figure S7.