Figure 4.

Breathing” Modulated Enzyme Catalytic Process in the Helicoidal Cisternae Stacks

(A and C) Fluorescence image of the helicoidal cisternae stack (A) and ellipsoidal liposome (C) encapsulated with Rhodamine B isothiocyanate-Dextran (∼70 kDa).

(B and D) The profile of the fluorescence intensity along the (B) white dashed line in (A) and (D) white dashed line in (C).

(E) Schematic illustration of the enzyme catalytic reactions in the helicoidal cisternae stacks and typical fluorescence images of the cisternae stacks in different states encapsulated with fluorescent products.

(F) Variation of the fluorescence intensity of the fluorescent product resorufin with time for cisternae stacks in expansion state (green curve) and compressed state (red curve), respectively.

(G) Variation of the maximum fluorescence intensity of the reaction product resorufin with cycle numbers in compressed (red) and expanded (green) cisternae stacks.

Scale bars, 10 μm. Error bars are ±SEM. See also Figures S20–S22.