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. 2018 Oct;188(10):2392–2405. doi: 10.1016/j.ajpath.2018.06.020

Figure 1.

Figure 1

Expression of kdPKCζ in mouse tissues. A: Plasmid map of Tek-driven kdPKCζ DNA construct with enhancer. B: Expression of kdPKCζ in mouse tissues by PCR; noncarrier (NC) versus kdPKCζ water or primer alone. C: Analysis of kdPKCζ expression in the retina by immunofluorescence. Six-week–old kdPKCζ carrier and NC littermate mice were analyzed for the expression of hemagglutinin (HA) tag (red) along with endothelial marker CD31 (green) and zonula occludens protein 1 (ZO-1; far red) in retinal cross sections. Yellow arrows indicate expression of kdPKCζ in retinal blood vessel. D: Expression of kdPKCζ in the circulating leukocytes of kdPKCζ mice but not NC controls. The product was observed only after cDNA synthesis and not from RNA alone, indicating no genomic contamination. Original magnification, ×630 (C). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; M, marker.