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. 2018 Oct;188(10):2392–2405. doi: 10.1016/j.ajpath.2018.06.020

Figure 4.

Figure 4

Expression of kdPKCζ attenuates ischemia-reperfusion (IR)–induced inflammation. Noncarrier (NC) and kdPKCζ mice were subjected to IR injury. At 48 hours after IR, retina samples were harvested for RNA isolation, followed by mRNA quantification by nCounter Analysis System using Immunology v2 kit. A: Heat map represents expression level from low (green) to high (red). B: Quantitative RT-PCR analysis of mRNA profile of various proinflammatory cytokines from a separate IR experiment with sample collection at 48 hours after injury. Every gene shown represents an increase after IR in the NC animals, which was significantly attenuated by expression of kdPKCζ, as determined by analysis of variance and Tukey's post hoc test. Data table and statistics are shown in Supplemental Table S1. C: Immunofluorescence staining of retinal cross section for CFB (green) and nuclei (blue) 48 hours after IR. Original magnification, ×200 (C). CCL, chemokine (C-C motif) ligand; CFB, complement factor B; CXCR, C-X-C chemokine receptor; ICAM, intercellular adhesion molecule; LFA, lymphocyte function-associated antigen; NOS, nitric oxide synthase; TNF, tumor necrosis factor.