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. 2018 Sep 4;10(10):e9155. doi: 10.15252/emmm.201809155

Figure EV5. Indirubin‐3‐monoxime (IR3mo) rescues the CCM phenotype in krit1 ty219c mutant zebrafish and CCM2‐ and CCM3‐depleted HUVECs.

Figure EV5

  • A–D
    Treatment with 5 μM IR3mo rescues the embryonic zebrafish krit1 ty219c mutant ballooning heart phenotype. Shown are images of confocal z‐scan projections of wild‐type (WT) (A, C) and krit1 ty219 mutant (B, D) zebrafish embryonic hearts at 48 hpf expressing the endothelial reporter Tg(kdrl:GFP)s843 (green) and counter‐stained for ACTIN (red). Scale bar is 50 μm.
  • E–J
    Treatment with 10 μM IR3mo for 48 h rescued the CCM phenotype in CCM2‐ or CCM3‐depleted HUVECs. Shown are confocal images of HUVECs with labeled VE‐cadherin (green) and F‐ACTIN (red). Control siRNA‐silenced (E, F), CCM2 siRNA‐silenced (G, H), or CCM3 siRNA‐silenced (I, J) HUVECs untreated (E, G, I) or treated (F, H, J) with IR3mo. Scale bar is 10 μm.