Table 1. Expression constructs generated in this study.
| Plasmid | Description a | GenBank Acc. No. |
| pSynMch1 | Synthetic A-type mch cluster b (P5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-T3mchCA) | MG583853 |
| pSynMch2 | Synthetic A-type mch cluster (P5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-T3mchCA) | MG583854 |
| pSynMch3 | Synthetic B-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBA-3BB5CB-mchCB-T3mchCB) | MG583855 |
| pSynMch4 | Synthetic C-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBA-3BC5CC-mchCC-T3mchCC) | MG583856 |
| pSynMch5 | Synthetic D-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBD-3BD5CD-mchCD-T3mchCD) | MG583857 |
| pSynMch6 | Synthetic S-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBS-3BS5CS-mchCS-T3mchCS) | MG583858 |
| pSynMch8 | Synthetic AS-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCS-T3mchCS) | MG583859 |
| pSynMch9 | Synthetic SA-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBS-3BA5CA-mchCA-T3mchCA) | MG583860 |
| pSynMch10 | Synthetic SC-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBS-3BC5CC-mchCC-T3mchCC) | MG583861 |
| pSynMch11 | Synthetic SB-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBS-3BB5CB-mchCB-T3mchCB) | MG583862 |
| pSynMch12 | Synthetic SD-type hybrid mch cluster (P5mchAA-mchAA-3AA5BA-mchBS-3BD5CD-mchCD-T3mchCD) | MG583863 |
| pSynMch13 | Synthetic A-type mch cluster (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-T3mchCA) | MG583864 |
| pSynMch14 | Synthetic SA-type hybrid mch cluster (PTn5mchAA-mchAA-3AA5BA-mchBS-3BA5CA-mchCA-T3mchCA) | MG583865 |
| pSynMch15 | Synthetic SB-type hybrid mch cluster (PTn5mchAA-mchAA-3AA5BA-mchBS-3BB5CB-mchCB-T3mchCB) | MG583866 |
| pSynMch16 | Synthetic SD-type hybrid mch cluster (PTn5mchAA-mchAA-3AA5BA-mchBS-3BD5CD-mchCD-T3mchCD) | MG583867 |
| pSynMch17 | Synthetic A-type mch cluster with inactivated CP1 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-CP1*-3BA5CA-mchCA-T3mchCA) | MG583868 |
| pSynMch18 | Synthetic A-type mch cluster with inactivated CP2 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-CP2*-3BA5CA-mchCA-T3mchCA) | MG583869 |
| pSynMch19 | Synthetic A-type mch cluster with inactivated CP3 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-CP3*-T3mchCA) | MG583870 |
| pSynMch20 | Synthetic A-type mch cluster with inactivated CP4 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-CP4.1*-T3mchCA) | MG583871 |
| pSynMch21 | Synthetic A-type mch cluster with inactivated CP4 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-CP4.2*-T3mchCA) | MG583872 |
| pSynMch22 | Synthetic A-type mch cluster with inactivated CP5 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-CP5*-T3mchCA) | MG583873 |
| pSynMch23 | Synthetic A-type mch cluster with inactivated CP6 domain (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA-CP6*-T3mchCA) | MG583874 |
| pSynMch24 | Synthetic S-type mch cluster with reactivation of CP4 domain (P5mchAA-mchAA-3AA5BA-mchBS-3BS5CS-mchCS-CP4-T3mchCS) | MG583875 |
| pSynMch25 | Synthetic A-type mch cluster with duplicated module 1 (PTn5mchAA-mchAA-3AA5BA-mchBA[duplM1]-3BA5CA-mchCA-T3mchCA) | MG583876 |
| pSynMch26 | Synthetic A-type mch cluster with duplicated module 2 (PTn5mchAA-mchAA-3AA5BA-mchBA[duplM2]-3BA5CA-mchCA-T3mchCA) | MG583877 |
| pSynMch27 | Synthetic A-type mch cluster with duplicated module 3 (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA[duplM3]-T3mchCA) | MG583878 |
| pSynMch28 | Synthetic A-type mch cluster with duplicated module 6 (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA[duplM6]-T3mchCA) | MG583879 |
| pSynMch29 | Synthetic A-type mch cluster with deletion of module 1 (PTn5mchAA-mchAA-3AA5BA-mchBA[delM1]-3BA5CA-mchCA-T3mchCA) | MG583880 |
| pSynMch30 | Synthetic A-type mch cluster with deletion of module 2 (PTn5mchAA-mchAA-3AA5BA-mchBA[delM2]-3BA5CA-mchCA-T3mchCA) | MG583881 |
| pSynMch31 | Synthetic A-type mch cluster with deletion of module 3 (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA[delM3]-T3mchCA) | MG583882 |
| pSynMch32 | Synthetic A-type mch cluster with deletion of module 4 (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA[delM4]-T3mchCA) | MG583883 |
| pSynMch33 | Synthetic A-type mch cluster with deletion of module 5 (PTn5mchAA-mchAA-3AA5BA-mchBA-3BA5CA-mchCA[delM5]-T3mchCA) | MG583884 |
a mch clusters in pSynMch1-12 are under the control of the native promoter, while in pSynMch13-33 mch clusters the PTn5 promoter (PnptII) was used. The types of gene cluster in the corresponding synthetic segments are illustrated in lowercase; modified positions are indicated in lowercase and bold.
bDesign of A-type mch cluster from M. xanthus DK1622 (GenBank Acc. No. KX622595) based on AarI restriction sites. Except this construct, the design of all other mch clusters based on BsaI restriction sites.