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Alanyl-AMB (2) is the product of in vitro reconstitution of amb enzymes. A) Extracted ion chromatograms (EICs) of 2 (m/z 203.1026 [M+H]+) from LC-HRMS in the one-pot assay and negative controls. B) Fragmentation pattern of Fmoc-Ala-AMB from tandem mass spectrometry. Fragments in red indicate that the alanine portion of Ala-AMB is attached to Fmoc. Complete analysis of all fragments is described in the Supporting Information, Figure S2.
