FIGURE 8:

Different degrees of PARP cleavage in fully transformed (3Y1^v-Src), partially transformed (3Y1^c-Src), and normal (3Y1) rat fibroblasts as indications of apoptosis. 3Y1, 3Y1^c-Src, and 3Y1^v-Src cells were treated with 8 µM P-Glu₄ for 24 h. Cells were irradiated at 0.84 mW cm⁻² for 3.5 min (1.76 kJ m⁻²), and 9 h later the cells were collected and lysed. The supernatant of the lysate was applied to Western blot to detect PARP cleavage. Lane 1: 3Y1 cells with no photodynamic treatment; lane 2: 3Y1^c-Src cells with no photodynamic treatment; lane 3: 3Y1^v-Src cells with no photodynamic treatment; lane 4: 3Y1 cells with irradiation but no P-Glu₄; lane 5: 3Y1^c-Src cells with irradiation but no P-Glu₄; lane 6: 3Y1^v-Src cells with irradiation but no P-Glu₄; lane 7: 3Y1 cells with P-Glu₄ but no irradiation; lane 8: 3Y1^c-Src cells with P-Glu₄ but no irradiation; lane 9: 3Y1^v-Src cells with P-Glu₄ but no irradiation; lane 10: 3Y1 cells with irradiation and P-Glu₄; lane 11: 3Y1^c-Src cells with irradiation and P-Glu₄; and lane 12: 3Y1^v-Src cells with irradiation and P-Glu₄.