Figure 2.

V3_VLat INs Project Both Ipsilaterally and Contralaterally in the Spinal Cord

(A) Green fluorescent dextran was applied unilaterally at L3 to label contralateral L2 ventral V3 INs. MNs were then recorded in L2 sections while photostimulating dextran-positive V3_VLat INs (orange).

(B) Representative images of dextran labeled spinal cord section used for recordings. (B1) Unilateral dextran application site with contralateral neuronal labeling. Scale bar, 100 μm. (B2) Contralateral dextran-positive neurons. (B3) tdTom-expressing V3 INs (red). (B4) Overlay of (B2) and (B3). White and black arrowheads denote dextran-positive V3_VLat INs and V3_VMed INs, respectively. White dashed line outlines ventral horn of spinal cord. Scale bar, 20 μm.

(C) Photostimulation of dextran-positive V3_VLat INs produced EPSPs in patched MNs.