Figure 6.
Local Spinal Layers between V3 INs and MNs
(A) Systematic investigation of V3VMed → V3VLat → MN connectivity. Image obtained during patch-clamp recordings. Letters B–D depict the order in which neurons were patched and stimulated to demonstrate the layered connectivity within a slice (n = 3). M, medial; L, lateral; D, dorsal; V, ventral.
(B) Direct photostimulation of the patched MN elicited one action potential per stimulus.
(C) Photostimulating a V3VLat IN elicited a single action potential per stimulus in the recorded MN.
(D) Photostimulating a V3VMed IN elicited EPSPs in the loose-patched V3VLat IN that was stimulated in (C).
Scale bars, 100 μm.