Figure 1. Verification of Sema3C R745A mutant expression and secretion. A, Sequencing results of the putative furin cleavage site 742RNRR745 of the wild-type Sema3C and R745A mutant. B, Sema3C and R745A mutant protein expression in transfected 293FT cell extracts and cell medium. C: control (transfected with empty vector cells); W: wild-type Sema3C; M: mutant Sema3C (R745A). Black arrows indicate Sema3C and R745A mutant proteins of 85.2 kDa in size.
