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. 2018 Oct 10;12:22. doi: 10.1186/s13036-018-0110-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a, b Single-factor analysis of basal culture medium revealed optimal carbon and nitrogen sources for producing therapeutic DNA vaccine pcDNA-CCOL2A1. a Evaluation of the optimal basal culture medium among LB, 2 × YT, TB, M9 (Glycerol) and M9 (Glucose) in shaking flask culture through One-Way ANOVA. b Screen the optimal carbon and nitrogen sources in shaking flask culture using One-Way ANOVA. *p < 0.05, * * p < 0.001. Data are expressed as the mean ± standard deviation (SD) of 3 independent experiments