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. 2018 Oct 10;12:22. doi: 10.1186/s13036-018-0110-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a, b, c Response surface 3D model (left) and contour plot (right) to assessthe effects of the three variables on the yield of plasmid DNA vaccine pcDNA-CCOL2A1 produced by the engineered E. coli DH5α. a response surface plot showing the mutual effect of peptone and mannitol on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; b response surface plot showing the mutual effect of peptone and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; c response surface plot showing the mutual effect of mannitol and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1