Table 5.
Through BBD optimizing the screened culture conditions for the yield of plasmid DNA vaccine pcDNA-CCOL2A1 by the engineered Escherichia coli DH5α
| Run | A-Peptone(g/L) | B-Mannitol(g/L) | C-Inoculum concentration(OD) | Plasmid yield (mg/L) |
|||
|---|---|---|---|---|---|---|---|
| Code level | Real level | Code level | Real level | Code level | Real level | ||
| 1 | 1 | 28 | −1 | 7 | 0 | 0.35 | 277.80 ± 8.38 |
| 2 | 1 | 28 | 0 | 8 | − 1 | 0.25 | 293.63 ± 5.63 |
| 3 | 1 | 28 | 0 | 8 | 1 | 0.45 | 307.20 ± 6.81 |
| 4 | 0 | 26 | −1 | 7 | 1 | 0.45 | 305.73 ± 9.32 |
| 5 | 0 | 26 | 1 | 9 | −1 | 0.25 | 305.33 ± 11.90 |
| 6 | 0 | 26 | 0 | 8 | 0 | 0.35 | 332.53 ± 14.61 |
| 7 | 0 | 26 | 0 | 8 | 0 | 0.35 | 335.60 ± 10.52 |
| 8 | 0 | 26 | 0 | 8 | 0 | 0.35 | 339.70 ± 10.91 |
| 9 | 0 | 26 | 0 | 8 | 0 | 0.35 | 345.10 ± 13.60 |
| 10 | 0 | 26 | −1 | 7 | −1 | 0.25 | 285.03 ± 8.23 |
| 11 | −1 | 24 | 0 | 8 | 1 | 0.45 | 309.53 ± 9.69 |
| 12 | −1 | 24 | 0 | 8 | −1 | 0.25 | 308.70 ± 10.68 |
| 13 | -1 | 24 | 1 | 9 | 0 | 0.35 | 295.80 ± 10.61 |
| 14 | 0 | 26 | 1 | 9 | 1 | 0.45 | 302.10 ± 14.45 |
| 15 | 1 | 28 | 1 | 9 | 0 | 0.35 | 310.73 ± 10.71 |
| 16 | 0 | 26 | 0 | 8 | 0 | 0.35 | 340.97 ± 13.16 |
| 17 | -1 | 24 | -1 | 7 | 0 | 0.35 | 304.93 ± 8.88 |
Data are expressed as the mean ± standard deviation (SD) of 3 batches independent experiments for each strain