Table 6.

Regression analysis of a full second-order polynomial model for the optimized yield of plasmid DNA vaccine by the engineered E. coli DH5α

Term	Sum of Squares	df	Mean Square	F-Value	p-value
Model	6344.09	9	704.90	46.72	<0.0001*
A	109.52	1	109.52	7.26	0.0309*
B	204.70	1	204.70	13.57	0.0078*
C	126.94	1	126.94	8.41	0.0230*
AB	442.39	1	442.39	29.32	0.0010*
AC	40.54	1	40.54	2.69	0.1452
BC	143.20	1	143.20	9.49	0.0178*
A2	1382.96	1	1382.96	91.65	<0.0001*
B2	2293.73	1	2293.73	152.01	<0.0001*
C2	1063.13	1	1063.13	70.46	<0.0001*
Residual	105.62	7	15.09
Lack of fit	10.92	3	3.64	0.15	0.9221
Pure Error	94.71	4	23.68
Cor Total	6449.71	16

R² = 0.9836, adj-R² = 0.9626, Adequate precision = 20.387, CV = 1.25%, Adequate precision = 20.387

*Identified variables with a significant effect on the response (p-value<0.05)