Figure 2.

Dis2 Is a Thr4P Phosphatase

(A) Deletion of dis2 or expression of the phosphatase-defective dis2-R245Q allele increase the level of Thr4P. Whole-cell extracts were analyzed by western blot using antibodies specific for Thr4P, Tyr1P, Ser2P, Ser5P, Ser7P, and Pol II (8WG16).

(B) Silver-stained SDS-PAGE analysis of Pol II, Dis2, and Dis2 R245Q purified from yeast.

(C) Purified Pol II was incubated with Dis2, and levels of phosphorylated Pol II were assessed by western blot using antibodies specific for Thr4P, Tyr1P, Ser2P, and Ser5P.

(D) Purified Pol II was incubated with Dis2 or Dis2 R245Q, and levels of Thr4P Pol II were assessed by western blot with anti-CTD and Flag antibodies.

See also Figure S1.