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. 2018 Oct 2;25(1):259–269.e5. doi: 10.1016/j.celrep.2018.09.007

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Lack of Dis2 Activity Leads to Global Decrease of the Termination Factor Seb1

(A–C) Spike-in normalized ChIP-seq meta-profiles of Pcf11 (A), Rhn1 (B), and Seb1 (C) around the PAS, averaged from two biological replicas. Protein-coding genes for analysis were selected as above (n = 1,735).

(D) Pcf11, Rhn1, and Seb1 profiles on individual genes.

(E) Binding of SUMO-Pcf11-CID to the FAM-tagged two-repeat non-phosphorylated or phosphorylated CTD peptides measured by FA. Error bars show the SD of two technical repeats.

(F) FA assay for Seb1-CID. Error bars show the SD of two technical repeats.

See also Figures S3 and S4 and Table S1.