Fig. 1.

Downsampling of cell number preserves major cell type distinctions. a t-SNE plots of the full dataset and five smaller downsampled subsets. Each dataset is shown in the t-SNE space of the full dataset. Clustering was performed independently in every subset. b Cluster preservation is a key metric to evaluate similarities and differences between clusters from different analyses, measuring preservation as a fraction of the original cluster that remains in analyzed subsets. The diagram depicts a simplified cluster preservation calculation (see also the “Methods” section). c Cluster preservation represents the best instance of the fraction of a cluster that is represented during downsampling. Nine original subsets are represented and a total of 56 datapoints are represented; the cell number is shown on a log₂ (number of cells) score to improve ease of graph interpretation