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. 2018 Oct 10;19:734. doi: 10.1186/s12864-018-5091-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Single-cell transcriptional profiles of in vivo embryos, NT embryos and donor cells. a PCA of all 60 samples revealing key relationships between groups. Smaller symbols represent each sample. Bigger symbols represent the average of samples from each group. b The number of genes displaying differential expression between adjacent developmental stages. c The number of genes exhibiting differential expression between different types of embryo at the same stage. d Heatmap of all 60 samples. Blue indicates lower expression and red indicates higher expression. The hierarchical clustering analysis of the samples is shown on top of the heatmap. The hierarchical clustering analysis of the genes is shown on the left. NTM_23 means the third sample of 2-cell NTM embryos. CC_1 means the first sample of cumulus cells, etc.