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. 2018 Oct 8;36(42):6270–6281. doi: 10.1016/j.vaccine.2018.09.008

Fig. 3.

Fig. 3

Mean frequencies (±SEM) of MNCs expressing (A) TLR2 and (B) TLR4, and (C) serum endotoxin levels in HIFM transplanted gnotobiotic pigs fed protein deficient diet (Deficient HIFM) and protein sufficient diet (Sufficient HIFM) groups. Mononuclear cells (MNCs) were isolated from blood, spleen, ileum, and duodenum of the piglets from Deficient HIFM and Sufficient HIFM groups pre- and post-virulent HRV challenge (PTD 24/PCD 0 and PTD 31/PCD 7, respectively). The mean serum endotoxin levels are shown with SEM. Significant difference (*p < 0.05) between Deficient HIFM and Sufficient HIFM groups or between pre- and post-virulent HRV challenge in each group were determined by Mann-Whitney U test. HRV; human rotavirus, Deficient; protein deficient diet, Sufficient; protein sufficient diet, HIFM; human infant fecal microbiota, PTD; post transplantation day, PVD; post 1st (2nd) vaccination day and PCD; post challenge day.